Vitamin D Testing: Is it 25-OH or 1,25-Dihydroxy?

Nam K. Tran, PhD, MS, FACB, Director of Clinical Chemistry and POCT;Dongguang Wei, MD, Pathology Resident
Introduction: Vitamin D represents a group of fat soluble sterols that help with the intestinal absorption of calcium, magnesium, phosphate, and zinc.1,2 These sterols include ergocalciferol D2, and cholecalciferol D3. Vitamin D is rapidly metabolized in the liver to form 25-hydroxy (OH) vitamin D. Further hydroxylation in the kidney under the influence of parathyroid hormone (PTH) produces 1,25-dihydroxyvitamin D. [...]

By | 2017-08-14T12:38:02+00:00 August 15, 2017|0 Comments

Best Practices for Cardiac Troponin Testing: Part I

Nam K. Tran, PhD, MS, FACB, Associate Professor and Director of Clinical Chemistry;Ezra Amsterdam, MD, Distinguished Professor, Cardiovascular Medicine;Stacey Howell, MD, Resident Physician, Internal Medicine;Bryn Mumma, MD, MAS, Assistant Professor, Emergency Medicine;Javier E. López, MD, MAS, Assistant Professor, Cardiovascular Medicine
Background: Cardiac troponin (cTn) is the preferred biomarker that aids in the diagnosis of acute myocardial infarction (MI) as defined by the 3rd Universal Definitions of MI.1 This month’s blog (Part 1 of 3) will review [...]

By | 2017-07-28T10:04:32+00:00 July 28, 2017|0 Comments

Biotin Interference in Clinical Immunoassays: The Dose Makes the Interference

Guofeng “George” Gao, MD, Resident Pathologist and Nam Tran, PhD, MS, FACB, Director of Clinical Chemistry and POCT
Background: Biotin, also known as Vitamin B7, is a co-factor in fatty acid metabolism, amino acid degradation, and gluconeogenesis. The recommended daily intake (RDI) for biotin is extremely low—about 30 µg/day. [...]

By | 2017-06-20T11:05:13+00:00 June 15, 2017|0 Comments

Modern Laboratory Techniques for Antiphospholipid Syndrome Testing

John Rodrigo, MD, Postdoctoral Scholar; Fabian Lara, MD, Postdoctoral Scholar; Saba Ali, MD, Resident Pathologist; Denis Dwyre, MD, Director of Hematopathology; Grace Monis, MD, Special Coagulation; Sarah Barnhard, MD, Associate Director of Transfusion; Scott Bainbridge, CLS, Special Chemistry/Toxicology Supervisor; and Nam Tran, PhD, Director of Clinical Chemistry and SARC
Background: Antiphospholipid syndrome (APS) is an autoimmune disorder [...]

By | 2017-06-20T10:45:28+00:00 May 15, 2017|0 Comments