Molecular Pathogen Detection at the Point of Care: Next Generation Flu Testing!

Nam K. Tran, PhD, MS, FACB, Director of Clinical Chemistry and POCT Larissa May, MD, MSPH, MSHS, Professor and Director of Emergency Department Antimicrobial Stewardship Shelley Gillot, CLS Specialist (POCT) Stacy Yee, CLS Specialist (POCT) Introduction For years, antigen-based rapid influenza diagnostic tests (RIDT) [...]

By | 2017-11-16T08:52:31+00:00 November 16, 2017|0 Comments

Best Practices for HIV-1/2 Screening: When to Test and What to Test

Ying Liu, MD, Resident Pathologist;Nam Tran, PhD, Director of Clinical Chemistry and SARC
Background: The World Health Organization (WHO) estimated by end of 2016 over 36.7 million people are living with human immunodeficiency virus (HIV) / acquired immunodeficiency syndrome (AIDS) and 1 million people die of AIDS annually.1 Early detection and treatment of the disease is instrumental in reducing the spread of HIV. There are two main methods for HIV testing: (a) immunoassay, and (b) molecular techniques. [...]

By | 2017-09-14T11:02:08+00:00 September 15, 2017|0 Comments

Vitamin D Testing: Is it 25-OH or 1,25-Dihydroxy?

Nam K. Tran, PhD, MS, FACB, Director of Clinical Chemistry and POCT;Dongguang Wei, MD, Pathology Resident
Introduction: Vitamin D represents a group of fat soluble sterols that help with the intestinal absorption of calcium, magnesium, phosphate, and zinc.1,2 These sterols include ergocalciferol D2, and cholecalciferol D3. Vitamin D is rapidly metabolized in the liver to form 25-hydroxy (OH) vitamin D. Further hydroxylation in the kidney under the influence of parathyroid hormone (PTH) produces 1,25-dihydroxyvitamin D. [...]

By | 2017-08-14T12:38:02+00:00 August 15, 2017|0 Comments

Best Practices for Cardiac Troponin Testing: Part I

Nam K. Tran, PhD, MS, FACB, Associate Professor and Director of Clinical Chemistry;Ezra Amsterdam, MD, Distinguished Professor, Cardiovascular Medicine;Stacey Howell, MD, Resident Physician, Internal Medicine;Bryn Mumma, MD, MAS, Assistant Professor, Emergency Medicine;Javier E. López, MD, MAS, Assistant Professor, Cardiovascular Medicine
Background: Cardiac troponin (cTn) is the preferred biomarker that aids in the diagnosis of acute myocardial infarction (MI) as defined by the 3rd Universal Definitions of MI.1 This month’s blog (Part 1 of 3) will review [...]

By | 2017-07-28T10:04:32+00:00 July 28, 2017|0 Comments

Biotin Interference in Clinical Immunoassays: The Dose Makes the Interference

Guofeng “George” Gao, MD, Resident Pathologist and Nam Tran, PhD, MS, FACB, Director of Clinical Chemistry and POCT
Background: Biotin, also known as Vitamin B7, is a co-factor in fatty acid metabolism, amino acid degradation, and gluconeogenesis. The recommended daily intake (RDI) for biotin is extremely low—about 30 µg/day. [...]

By | 2017-06-20T11:05:13+00:00 June 15, 2017|0 Comments